Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	956,860	C→T	K65K (AAG→AAA)	ycaO ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	956,860	0	C	T	76.9%	18.1 / 2.8	13	K65K (AAG→AAA)	ycaO	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (10/0);  total (12/1)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

TTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGCCAGCGCCGCTTTCTTGGTTGCGCCTTTACCGTTGGTAAAACACAGTGCGCACTCTTTGTC  >  W3110S.gb/956811‑956907                                                  |                                                ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:1627470/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:1718719/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:1787452/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:2140091/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:240314/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:2476406/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:2798503/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:2942967/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:823422/1‑61 (MQ=255) ttgAGAGACGCTCGAAATATTCACCGAGGGCAGAAGCCAGCGCCGCTTTTTTGGTTGCGcc                                      >  1:8366/1‑61 (MQ=255)               aaaTATTCACCGAGTGCAGAAGCCAGCGCCGCTTTCTTGGTTGCGCCTTTACCGTTGGTaa                        >  1:2861583/1‑61 (MQ=255)               aaaTATTCACCGAGTGCAGAAGCCAGCGCCGCTTTCTTGGTTGCGCCTTTACCGTTGGTaa                        >  1:927785/1‑61 (MQ=255)                                   aGCCAGCGCCGCTTTCTTGGTTGCGCC‑TTACCGTTGGTAAAACACAGTGCGCACTCTTTGTc  <  1:419787/62‑1 (MQ=255)                                                  |                                                TTGAGAGACGCTCGAAATATTCACCGAGTGCAGAAGCCAGCGCCGCTTTCTTGGTTGCGCCTTTACCGTTGGTAAAACACAGTGCGCACTCTTTGTC  >  W3110S.gb/956811‑956907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑