Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	997,358	G→C	N3K (AAC→AAG)	ssuA ←	alkanesulfonate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	997,358	0	G	C	92.9%	82.3 / ‑1.1	28	N3K (AAC→AAG)	ssuA	alkanesulfonate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (26/0);  total (26/2)
Fisher's exact test for biased strand distribution p-value = 2.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GCGCCAGTTTAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAG  >  W3110S.gb/997342‑997396                 |                                       gcgccagTTTAATGATCTTACGCATGGGCATTACCt                     >  1:1744169/1‑36 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2378848/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:936686/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:89781/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:785943/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:566779/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:529502/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:468118/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:458435/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2876289/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2741057/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2496975/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2396227/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2392814/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:1033326/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:212021/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2090597/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2089917/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2077772/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2072493/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:2036533/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:1740509/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:1593264/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:1551114/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:1518999/1‑37 (MQ=255) gcgccagTTTAATGATCTTACGCATGGGCATTACCTc                    >  1:107619/1‑37 (MQ=255)        tttAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAg  <  1:2065083/48‑1 (MQ=255)        tttAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAg  <  1:691481/48‑1 (MQ=255)                 |                                       GCGCCAGTTTAATGATGTTACGCATGGGCATTACCTCGCAGAGACAGAAGGTCAG  >  W3110S.gb/997342‑997396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑