| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 424,255 | C→T | S7S (TCC→TCT) | queA → | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 424,255 | 0 | C | T | 78.8% | 62.1 / 13.4 | 33 | S7S (TCC→TCT) | queA | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base T (0/26); total (5/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.85e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAAC > W3110S.gb/424200‑424301 | aCTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGaa > 1:1270685/1‑62 (MQ=255)aCTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGaa > 1:2063297/1‑62 (MQ=255)aCTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGaa > 1:2008089/1‑62 (MQ=255) tCATGCGCGTTACCTATTTCTCTTTTGAATTGCCCg < 1:1461911/36‑1 (MQ=37) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:485705/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2613069/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2645045/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2865839/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2925059/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:302912/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:377675/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:117973/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:597409/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:784202/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:879908/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:92833/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:954966/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:959094/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2498380/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2310671/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:2204879/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1902742/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1718431/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1458089/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1457084/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1410395/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1298076/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1231567/36‑1 (MQ=255) tCATGCGCGTTACCGATTTCTCTTTTGAATTGCCCg < 1:1164817/36‑1 (MQ=255) cgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCAc > 1:2340557/1‑46 (MQ=255) cgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCAc > 1:2071679/1‑46 (MQ=255) cgTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAAc < 1:546611/62‑1 (MQ=255) gTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAAc < 1:447367/61‑1 (MQ=255) | ACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAAC > W3110S.gb/424200‑424301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |