Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,303,633	G→T	Q246Y (CAG→TAT)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,303,633	0	G	T	100.0%	87.2 / NA	28	Q246Y (CAG→TAT)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/28);  total (0/28)

GTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGA  >  W3110S.gb/1303580‑1303659                                                      |                           gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:1288050/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:1429757/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:1811027/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:181137/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:2006453/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:2934651/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:2811081/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:279505/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:2794183/62‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTAGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                    <  1:565087/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:1297932/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:688758/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:685097/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:422827/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:3120471/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:2861124/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:2707306/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:2445564/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:2342391/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:1823832/62‑1 (MQ=255)                   aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:1641309/62‑1 (MQ=255)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:2490918/36‑1 (MQ=37)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:2878515/36‑1 (MQ=37)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:2419549/36‑1 (MQ=37)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:1914179/36‑1 (MQ=37)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:895878/36‑1 (MQ=37)                          ggAACAACGCGAAAACCGTTATTAACTATGTAACCt                     <  1:989837/36‑1 (MQ=37)                                             tattaACTATGTAACCTATTTGCCTATTGCTTCTGa  <  1:2376676/36‑1 (MQ=37)                                                      |                           GTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGA  >  W3110S.gb/1303580‑1303659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑