Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,359,506	C→G	intergenic (‑124/+10)	ymjA ← / ← puuP	hypothetical protein/putrescine importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,359,506	0	C	G	87.5%	16.4 / ‑3.0	8	intergenic (‑124/+10)	ymjA/puuP	hypothetical protein/putrescine importer
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (7/0);  total (8/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTATT  >  W3110S.gb/1359449‑1359517                                                          |            cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:1186835/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:1230251/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:1235431/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:1276896/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:2588481/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:437985/1‑61 (MQ=255) cATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGGATc          >  1:971241/1‑61 (MQ=255)        ttATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTAtt  >  1:3045856/1‑62 (MQ=255)                                                          |            CATCTTTTTATAGTTAATGGCGCGGCGCGTTACCCTCAGGCAGGATAATGCGCCGCGCATCCGACTATT  >  W3110S.gb/1359449‑1359517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑