Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,383,164 C→T D210D (GAC→GAT)  ycjV → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,383,1640CT75.8% 54.5 / 16.7 34D210D (GAC→GAT) ycjVpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base T (25/0);  total (29/5)
Fisher's exact test for biased strand distribution p-value = 2.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAAACCGTTTATAACCAACCCGCGAAT  >  W3110S.gb/1383114‑1383218
                                                  |                                                      
gATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCa                                             <  1:2332416/62‑1 (MQ=255)
gATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCa                                             <  1:2355991/62‑1 (MQ=255)
gATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCa                                             <  1:1659030/62‑1 (MQ=255)
gATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCa                                             <  1:2430459/62‑1 (MQ=255)
gATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCa                                             <  1:2749202/62‑1 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2739244/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2334252/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2415020/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:254349/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2627026/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:271515/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:963217/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2925891/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2999832/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:3049202/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:335543/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:799735/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1062526/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2320638/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2269369/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2145760/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1998699/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1872924/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1856532/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1776931/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1703676/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1618667/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1498339/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1426594/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGATGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:1351876/1‑61 (MQ=255)
               aTGACCATGGCGACGCGGATTGTCATTATGAAGGAGGGGATTGTTCAGCAGGTCGGTgcgc                               >  1:2183666/1‑61 (MQ=255)
                                           tGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAAACCGTTTATAACCAACCCGCGAat  >  1:2070313/1‑62 (MQ=255)
                                           tGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAAACCGTTTATAACCAACCCGCGAat  >  1:1741769/1‑62 (MQ=255)
                                           tGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAAACCGTTTATAACCAACCCGCGAat  >  1:3072101/1‑62 (MQ=255)
                                                  |                                                      
GATCAGACCGAAGCGATGACCATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAAACCGTTTATAACCAACCCGCGAAT  >  W3110S.gb/1383114‑1383218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑