Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,387,938	A→G	L238L (TTA→TTG)	ycjF →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,387,938	0	A	G	77.3%	38.9 / 7.1	22	L238L (TTA→TTG)	ycjF	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base G (17/0);  total (17/5)
Fisher's exact test for biased strand distribution p-value = 3.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGTCGATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTGG  >  W3110S.gb/1387897‑1387975                                          |                                      ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAatcgcat                            >  1:2256095/2‑53 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATGGCCACGc                    >  1:2521816/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:119333/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:521747/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:437483/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2964286/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2658135/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2460798/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2207990/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2030934/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:1920178/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:1822698/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:1717371/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:1584708/2‑61 (MQ=255) ctggtggATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:1530619/2‑61 (MQ=255) ctggtggATATGGCGTATATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:2007337/2‑61 (MQ=255) ctggtggATATGGAGTTTATCGCCTGGCGCAATCTGCGTTTGATTAATCGCATCGCCACGc                    >  1:255616/2‑61 (MQ=255)                  tATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTgg  <  1:2065903/62‑1 (MQ=255)                  tATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTgg  <  1:2171527/62‑1 (MQ=255)                  tATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTgg  <  1:2399261/62‑1 (MQ=255)                  tATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTgg  <  1:1819030/62‑1 (MQ=255)                  tATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTgg  <  1:1117838/62‑1 (MQ=255)                                          |                                      TTGGTCGATATGGCGTTTATCGCCTGGCGCAATCTGCGTTTAATTAATCGCATCGCCACGCTGTATGGCATTGAACTGG  >  W3110S.gb/1387897‑1387975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑