Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,614,569	C→A	A133S (GCA→TCA)	yneH ←	predicted glutaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,614,569	0	C	A	81.2%	22.7 / 4.5	16	A133S (GCA→TCA)	yneH	predicted glutaminase
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (13/0);  total (16/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CACCGCTTAAGCCGCGCACGACTTCCAGCATACGTTGCCGTGGTGCGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAC  >  W3110S.gb/1614524‑1614610                                              |                                          cacCGCTTAAGCCGCGCACGACTTCCAGCATACGTTGCCGTGGTGCGCtt                                       >  1:1500444/1‑50 (MQ=255) cacCGCTTAAGCCGCGCACGACTTCCAGCATACGTTGCCGTGGTGCGCtt                                       >  1:2563472/1‑50 (MQ=255) cacCGCTTAAGCCGCGCACGACTTCCAGCATACGTTGCCGTGGTGCGCtt                                       >  1:2593356/1‑50 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGCCCACCAGCGCAc  >  1:419208/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCa   >  1:1534155/1‑61 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:1096016/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:1199249/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:1418464/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:2048515/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:2139864/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:2150564/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:2312825/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:2346821/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:283124/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:431678/1‑62 (MQ=255)                          cAGCATACGTTGCCGTGGTGAGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAc  >  1:631119/1‑62 (MQ=255)                                              |                                          CACCGCTTAAGCCGCGCACGACTTCCAGCATACGTTGCCGTGGTGCGCTTAATCGCCCTTGCAACATATCGCAGACCACCAGCGCAC  >  W3110S.gb/1614524‑1614610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑