| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 455,022 | C→T | H222H (CAC→CAT) | tig → | peptidyl‑prolyl cis/trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 455,022 | 0 | C | T | 85.7% | 48.1 / 1.7 | 21 | H222H (CAC→CAT) | tig | peptidyl‑prolyl cis/trans isomerase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (0/18); total (1/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAA > W3110S.gb/454975‑455079 | gCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGaa > 1:2700391/1‑62 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAGGGTAAAGCAGCGAAATTCGCTATCaa < 1:2743038/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2564402/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:801964/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:768241/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:720161/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:608657/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:477047/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:3033666/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2965721/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2582843/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1020839/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2496711/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2284353/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1834038/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1670020/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1658721/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1414128/62‑1 (MQ=255) aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1224158/62‑1 (MQ=255) aCCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGaa < 1:2036979/62‑1 (MQ=255) aCCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGaa < 1:1538485/62‑1 (MQ=255) | GCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAA > W3110S.gb/454975‑455079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |