| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,616,734 | G→A | A73T (GCG→ACG) | yneJ → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,616,734 | 0 | G | A | 76.9% | 43.5 / 11.0 | 26 | A73T (GCG→ACG) | yneJ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (20/0); total (24/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
TCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATG > W3110S.gb/1616691‑1616773 | tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAgc < 1:806067/60‑1 (MQ=255)tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAgc < 1:3087141/60‑1 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGc > 1:388609/1‑56 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGc > 1:1866304/1‑56 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2188775/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:593798/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:393273/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:3042466/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2906534/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2815880/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2391326/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2391167/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2139894/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2128187/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:2111613/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1923290/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1604622/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1390324/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1366326/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1308419/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1274908/1‑57 (MQ=255) aTAACTTTTTACGCTATAGCCAACAAATTCTCACGTTAGTGGATGAAGCGCGGAGCg > 1:1252843/1‑57 (MQ=255) aCGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATg > 1:1188212/1‑62 (MQ=255) aCGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATg > 1:2753201/1‑62 (MQ=255) aCGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATg > 1:1470122/1‑62 (MQ=255) aCGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATg > 1:39256/1‑62 (MQ=255) | TCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATG > W3110S.gb/1616691‑1616773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |