Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,621,230	G→A	A133T (GCA→ACA)	marR →	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,621,230	0	G	A	100.0%	76.4 / NA	25	A133T (GCA→ACA)	marR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/17);  total (8/17)

CCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACG  >  W3110S.gb/1621193‑1621287                                      |                                                          ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGa                                   <  1:2849163/62‑1 (MQ=255) ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGa                                   <  1:2396188/62‑1 (MQ=255) ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGa                                   <  1:646420/62‑1 (MQ=255) ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGa                                   <  1:836394/62‑1 (MQ=255) ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGa                                   <  1:2321822/62‑1 (MQ=255) ccAAGAATTAACAAAAAACCTGACGGCGGACGAAGGGACAACACTTGAGTATTTGCTTAAGa                                   <  1:401295/62‑1 (MQ=255)        ttAACAAAAAACCTGACGGCTGACGAAGTGACAACACTTGAGTATTTGCTTATGAAAGTCCt                            >  1:747019/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTcc                             >  1:453921/1‑61 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:1959414/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:1689173/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:1491378/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:1337758/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:3060024/1‑62 (MQ=255)        ttAACAAAAAACCTGACGGCGGACGAAGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCt                            >  1:919545/1‑62 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:2598460/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:794719/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:4540/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:350531/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:3004010/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:1069675/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:233366/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:209516/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:1825468/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:1710246/62‑1 (MQ=255)                                  aGTGACAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg  <  1:1290308/62‑1 (MQ=255)                                      |                                                          CCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACG  >  W3110S.gb/1621193‑1621287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑