Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,659,538	C→T	A87V (GCT→GTT)	ynfD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,659,538	0	C	T	100.0%	50.3 / NA	17	A87V (GCT→GTT)	ynfD	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/6);  total (11/6)

GCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGA  >  W3110S.gb/1659494‑1659595                                             |                                                          gCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGTTCCCGCGCAGTCTAGcc                                          >  1:2431759/1‑62 (MQ=255) gCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGTTCCCGCGCAGTCTAGc                                           >  1:996618/1‑61 (MQ=255)                            caGCGGTAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCTAACCGCAGt               <  1:411568/62‑1 (MQ=255)                            caGCGGTAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGt               <  1:1657963/62‑1 (MQ=255)                            caGCGGTAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGt               <  1:1918837/62‑1 (MQ=255)                                 gTAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGt               <  1:3095266/57‑1 (MQ=255)                                 gTAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAacac          <  1:2472053/62‑1 (MQ=255)                                  tAACGTCTCTGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAacac          <  1:1610665/61‑1 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGGCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:2973515/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:2477692/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:98526/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:321042/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:2603342/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:255281/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:2423677/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:2216334/1‑61 (MQ=255)                                          ctGTTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa  >  1:1653155/1‑61 (MQ=255)                                             |                                                          GCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGA  >  W3110S.gb/1659494‑1659595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑