Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,687,470	T→C	H278R (CAC→CGC)	fumC ←	fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,687,470	0	T	C	100.0%	37.4 / NA	12	H278R (CAC→CGC)	fumC	fumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/12);  total (0/12)

CATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATC  >  W3110S.gb/1687420‑1687489                                                   |                    catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1149732/62‑1 (MQ=255) catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1389902/62‑1 (MQ=255) catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1418504/62‑1 (MQ=255) catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:208791/62‑1 (MQ=255) catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:815379/62‑1 (MQ=255)  atcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1024895/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1048071/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1603157/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1866573/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:2125734/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:2595866/61‑1 (MQ=255)          cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:628360/61‑1 (MQ=255)                                                   |                    CATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATC  >  W3110S.gb/1687420‑1687489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑