Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,754,714	G→A	G277G (GGC→GGT)	ydhV ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,754,714	0	G	A	100.0%	50.2 / NA	17	G277G (GGC→GGT)	ydhV	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/11);  total (6/11)

GCCGCCGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGC  >  W3110S.gb/1754655‑1754758                                                            |                                             gccgccGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTAcc                                            >  1:1340089/1‑62 (MQ=255) gccgccGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTAcc                                            >  1:2989382/1‑62 (MQ=255) gccgccGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTAcc                                            >  1:2811163/1‑62 (MQ=255) gccgccGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTAcc                                            >  1:2473909/1‑62 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:890008/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:3075221/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:2990214/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:263328/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:2391367/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:2124012/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:1755188/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:1513615/62‑1 (MQ=255)                tCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCAcc                             <  1:1177512/62‑1 (MQ=255)                     aaCGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCACCCGtt                         >  1:1609118/1‑61 (MQ=255)                        ggATTTATAGGTACGAAAGCCGACCGTATTCTGATTACCTGGCGGAATTTCACCCGTTTCaa                     >  1:3114766/1‑62 (MQ=255)                                           ccGACCGTATTCTGATTACCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCcgc  <  1:3064566/62‑1 (MQ=255)                                                         aTTACCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCcgc  <  1:1045891/48‑1 (MQ=255)                                                            |                                             GCCGCCGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGC  >  W3110S.gb/1754655‑1754758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑