Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,756,242	T→C	intergenic (‑51/+404)	ydhY ← / ← ydhZ	predicted 4Fe‑4S ferridoxin‑type protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,756,242	0	T	C	100.0%	63.3 / NA	19	intergenic (‑51/+404)	ydhY/ydhZ	predicted 4Fe‑4S ferridoxin‑type protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/13);  total (6/13)

GGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGATATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAGATATTTTC  >  W3110S.gb/1756185‑1756296                                                          |                                                       ggTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    <  1:663681/62‑1 (MQ=255) ggTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    <  1:1841526/62‑1 (MQ=255) ggTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    <  1:282020/62‑1 (MQ=255)  gTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    <  1:1564958/61‑1 (MQ=255)  gTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    >  1:1956643/1‑61 (MQ=255)  gTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGACATTa                                                    >  1:2760124/1‑61 (MQ=255)                                acaGTGCTGACAATTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTc                     >  1:787148/1‑62 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:2068696/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:748085/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:2889176/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:2447403/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:189669/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:1571784/62‑1 (MQ=255)                                           aaTTCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:1440572/62‑1 (MQ=255)                                             ttCAGCAGCCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTc                     >  1:1439686/1‑49 (MQ=255)                                                   agcCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:1320527/54‑1 (MQ=255)                                                   agcCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAGATAtttt   >  1:2968793/1‑61 (MQ=255)                                                   agcCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAGATATTTTc  >  1:2771403/1‑62 (MQ=255)                                                    gcCTGACATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAg          <  1:1915466/53‑1 (MQ=255)                                                          |                                                       GGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAGCAGCCTGATATTAAGCATTCAACTAATACGCTGCTGACTCTGTCCCCAACACCAGATATTTTC  >  W3110S.gb/1756185‑1756296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑