Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,813,151	G→A	L168L (TTG→TTA)	ydjN →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,813,151	0	G	A	100.0%	47.1 / NA	16	L168L (TTG→TTA)	ydjN	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/0);  total (16/0)

ACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCG  >  W3110S.gb/1813105‑1813199                                               |                                                 aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:1323300/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2099139/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2156566/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2211644/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2708072/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2889025/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:2901741/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:3085623/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:397098/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:528777/1‑62 (MQ=255) aCTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGaaa                                   >  1:787221/1‑62 (MQ=255) aCTATGTTGGTAAAATCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATGCCGaaa                                   >  1:476997/1‑62 (MQ=255)             aaGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGAAAAACCCGtt                           >  1:1142465/1‑58 (MQ=255)             aaGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTATCCTTTATCCCGAAAAACCCGtt                           >  1:895902/1‑58 (MQ=255)                                        tGGTCTTATCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCg  >  1:2831607/1‑56 (MQ=255)                                        tGGTCTTATCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCg  >  1:3102448/1‑56 (MQ=255)                                               |                                                 ACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCG  >  W3110S.gb/1813105‑1813199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑