Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,824,512	C→A	A114E (GCG→GAG)	nadE →	NAD synthetase, NH3/glutamine‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,824,512	0	C	A	100.0%	74.8 / NA	24	A114E (GCG→GAG)	nadE	NAD synthetase, NH3/glutamine‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/11);  total (13/11)

TTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCA  >  W3110S.gb/1824464‑1824546                                                 |                                   ttGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGc                       <  1:1467041/62‑1 (MQ=255) ttGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGc                       <  1:1593317/62‑1 (MQ=255) ttGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGc                       <  1:1613673/62‑1 (MQ=255) ttGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGc                       <  1:1863356/62‑1 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:1080586/1‑61 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:1401354/1‑61 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:1516246/1‑61 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:2857918/1‑61 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:2054034/1‑61 (MQ=255)   gCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGAGGTATTGGCCAGCg                      >  1:2061381/1‑61 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:1241343/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:3010788/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:300005/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:2836455/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:2286781/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:2188537/1‑46 (MQ=255)                                  ttAATATCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGa      >  1:1556788/1‑46 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:2713318/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:1962335/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:1846690/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:1453729/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:589966/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:754114/44‑1 (MQ=255)                                        tCAAGGGCGAGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCa  <  1:911838/44‑1 (MQ=255)                                                 |                                   TTGCCTTTATTCAACCGGATCGCGTATTAACCGTTAATATCAAGGGCGCGGTATTGGCCAGCGAACAGGCATTGCGGGAAGCA  >  W3110S.gb/1824464‑1824546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑