| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 458,686 | G→A | R192H (CGT→CAT) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 458,686 | 0 | G | A | 86.3% | 126.3 / 12.4 | 51 | R192H (CGT→CAT) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base G (0/7); new base A (0/44); total (0/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
ACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCG > W3110S.gb/458639‑458717 | agtGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2914875/60‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:1835050/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2326629/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2154114/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2567645/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2596487/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:1969280/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:1960071/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2625435/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2921932/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:786960/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:802381/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:861539/62‑1 (MQ=255)aCTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2858038/62‑1 (MQ=255) cTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:2120723/61‑1 (MQ=255) cTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:325467/61‑1 (MQ=255) cTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:866888/61‑1 (MQ=255) aaCAGTCTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGa < 1:3005020/51‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTTGAATATCTGATGGCAATGATGGAATCg < 1:745212/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:2148901/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:2241991/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:219858/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:448623/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:70592/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:1332114/62‑1 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATGGAATCg < 1:1207719/62‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:359839/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2575144/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2995067/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:575279/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:630173/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2830620/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2139113/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:1146632/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:145653/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:1628052/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:1691767/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:1727766/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2815795/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2392450/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2452309/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:1022113/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:2694898/56‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACATCTGGAATATCTGATGGCAATGATgg < 1:273316/56‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATTTCt < 1:1620166/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:247444/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:1985293/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:1769512/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:695516/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2929589/35‑1 (MQ=255) gagaTGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2664692/35‑1 (MQ=255) | ACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCG > W3110S.gb/458639‑458717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |