| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,834,199 | G→A | A20T (GCC→ACC) | xthA → | exonuclease III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,834,199 | 0 | G | A | 100.0% | 60.8 / NA | 21 | A20T (GCC→ACC) | xthA | exonuclease III |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/13); total (8/13) | |||||||||||
TGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAGCCATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTC > W3110S.gb/1834150‑1834253 | tGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCAtc > 1:8462/1‑55 (MQ=255)tGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGaaa > 1:616306/1‑62 (MQ=255)tGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGaaa > 1:569868/1‑62 (MQ=255)tGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGaaa > 1:327454/1‑62 (MQ=255) aaCGGCCTGCGCTCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1879642/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1170776/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:777024/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:560233/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:365954/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:2193961/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1710026/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1697251/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1310878/50‑1 (MQ=255) aaCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1087393/50‑1 (MQ=255) aCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1961465/49‑1 (MQ=255) aCGGCCTGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCa < 1:1379021/49‑1 (MQ=255) tGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCACCAACCGGATGTGATTGGc > 1:1892720/1‑62 (MQ=255) tGCGCGCCAGACCTCACCAGCTTGAAACCATCGTCGAAAAGCACCAACCGGATGTGATTGGc > 1:1712861/1‑62 (MQ=255) gCTTGAAACCATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTc > 1:1903269/1‑62 (MQ=255) gCTTGAAACCATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTc > 1:2368784/1‑62 (MQ=255) gCTTGAAACCATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTc < 1:2960710/62‑1 (MQ=255) | TGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAGCCATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTC > W3110S.gb/1834150‑1834253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |