| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,842,398 | T→C | V406V (GTT→GTC) | ynjE → | predicted thiosulfate sulfur transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,842,398 | 0 | T | C | 85.7% | 12.4 / ‑3.4 | 7 | V406V (GTT→GTC) | ynjE | predicted thiosulfate sulfur transferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (6/0); total (7/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACCTTTATGTACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGT > W3110S.gb/1842358‑1842451 | aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTa > 1:1282513/1‑49 (MQ=255)aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTACGACGGTGGCTgg > 1:1300690/1‑62 (MQ=255)aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTACGACGGTGGCTgg > 1:1637289/1‑62 (MQ=255)aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTACGACGGTGGCTgg > 1:1770652/1‑62 (MQ=255)aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTACGACGGTGGCTgg > 1:2007200/1‑62 (MQ=255)aaaCCTTTATGTACGCACGCGCAATGGGCTGGAATAACGTCTCCGTTTACGACGGTGGCTgg > 1:2027729/1‑62 (MQ=255) agaaTGTTTCCGTGTATGACGGCGGCTGGTACGAATTGAGCAGCGATCCAAAAAATCCGGt > 1:2809089/1‑61 (MQ=255) | AAACCTTTATGTACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGT > W3110S.gb/1842358‑1842451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |