Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,855,467	G→A	T76I (ACT→ATT)	ydjE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,855,467	0	G	A	100.0%	102.6 / NA	32	T76I (ACT→ATT)	ydjE	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/23);  total (9/23)

TTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAA  >  W3110S.gb/1855417‑1855525                                                   |                                                           ttATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCg                                                 <  1:907510/62‑1 (MQ=255) ttATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCg                                                 <  1:2889366/62‑1 (MQ=255)       gAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCAACCAATAAGTGAGCCGATGaa                                            >  1:611511/1‑61 (MQ=255)        aaaCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAAt                                          <  1:1837593/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2328454/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:899670/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:591310/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2696604/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:261973/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2312626/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2312155/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2219055/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2084105/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:1446641/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:1528336/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:1601278/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAAcc                                      <  1:2012672/62‑1 (MQ=255)            gCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAATAAGAGAGCCGATGAAATAAcc                                      <  1:2616804/62‑1 (MQ=255)                   gccgccCAAAGTAGTCACCAATAAACCCACCTATAAGTGAGCCGATGAAATAAcc                                      <  1:1872651/55‑1 (MQ=255)                        ccAAAGTAGTCACCAATAAACCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGc                          <  1:1234009/62‑1 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCaaa                                   >  1:106798/1‑35 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggcggc           >  1:698406/1‑59 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:1708933/1‑60 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:2661054/1‑60 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:3067672/1‑60 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:308999/1‑60 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:41267/1‑60 (MQ=255)                                          aaCCCACCAATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCa          >  1:1468593/1‑60 (MQ=255)                                                ccaATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa  <  1:2727779/62‑1 (MQ=255)                                                ccaATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa  <  1:1500731/62‑1 (MQ=255)                                                ccaATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa  <  1:678470/62‑1 (MQ=255)                                                ccaATAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa  <  1:2216866/62‑1 (MQ=255)                                                   |                                                           TTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAA  >  W3110S.gb/1855417‑1855525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑