Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,870,352	T→C	Y577Y (TAT→TAC)	yeaG →	conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,870,352	0	T	C	84.6%	27.2 / ‑0.7	13	Y577Y (TAT→TAC)	yeaG	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (11/0);  total (12/1)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAA  >  W3110S.gb/1870305‑1870393                                                |                                          cgcgcCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCAc                             >  1:552941/1‑62 (MQ=255)                            aaTCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCaa  <  1:2495472/62‑1 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTtctcga   >  1:2782153/1‑50 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:125380/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:170733/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:1978902/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:2134898/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:2510989/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:2763785/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:2783941/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:3098013/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:416443/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa  >  1:612996/1‑53 (MQ=255)                                                |                                          CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAA  >  W3110S.gb/1870305‑1870393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑