Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,872,787	C→T	P230L (CCG→CTG)	yeaI →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,872,787	0	C	T	100.0%	24.6 / NA	9	P230L (CCG→CTG)	yeaI	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/0);  total (9/0)

TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCA  >  W3110S.gb/1872755‑1872816                                 |                              tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:1135751/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:1415526/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:1475586/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:1593749/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:1843738/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:208131/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:2510785/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:33994/1‑62 (MQ=255) tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa  >  1:459661/1‑62 (MQ=255)                                 |                              TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCA  >  W3110S.gb/1872755‑1872816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑