Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	540,240	T→A	F151Y (TTC→TAC)	ybbY →	predicted uracil/xanthine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	540,240	0	T	A	83.3%	24.3 / 0.1	12	F151Y (TTC→TAC)	ybbY	predicted uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (10/0);  total (12/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCA  >  W3110S.gb/540180‑540294                                                             |                                                       cGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCttttt                                                        <  1:1141912/61‑1 (MQ=255) cGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCttttt                                                        <  1:1588573/61‑1 (MQ=255) cGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCttttt                                                        <  1:1745670/61‑1 (MQ=255) cGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCttttt                                                        <  1:1938721/61‑1 (MQ=255)                      ttATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCg                                  >  1:695923/1‑62 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:1166538/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:1183378/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:1744469/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:1846235/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:2069562/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:2327189/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:2627691/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:320976/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:508301/1‑60 (MQ=255)                             gATGCTGGGCGCGCAGCTGACCACTATCTTTTACAAAGGTATGCTCGGGCTGCCGTTTgg                             >  1:750505/1‑60 (MQ=255)                                                       tCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCa  >  1:1476139/1‑61 (MQ=255)                                                             |                                                       CGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCA  >  W3110S.gb/540180‑540294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑