Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,984,357	C→T	L56L (TTG→TTA)	otsB ←	trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,984,357	0	C	T	100.0%	69.4 / NA	22	L56L (TTG→TTA)	otsB	trehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/4);  total (18/4)

GCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAG  >  W3110S.gb/1984300‑1984401                                                          |                                             gCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATcaatgctaatg                                          <  1:1692825/62‑1 (MQ=255) gCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATcaatgctaatg                                          <  1:2397712/62‑1 (MQ=255)        ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc                                   >  1:2804524/1‑62 (MQ=255)        ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc                                   >  1:1759619/1‑62 (MQ=255)        ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc                                   >  1:1768852/1‑62 (MQ=255)        ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc                                   >  1:1115616/1‑62 (MQ=255)        ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc                                   >  1:2441819/1‑62 (MQ=255)                                    ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt       <  1:1133535/62‑1 (MQ=255)                                    ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt       <  1:493112/62‑1 (MQ=255)                                    ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt       <  1:1624331/62‑1 (MQ=255)                                    ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt       <  1:25272/62‑1 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:2228492/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:921273/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:682168/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:2717383/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:2379524/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:2034671/1‑61 (MQ=255)                                          gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:1124963/1‑61 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:2356187/1‑54 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:1763543/1‑54 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:1750330/1‑54 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:1531066/1‑54 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:569139/1‑54 (MQ=255)                                                 tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg  >  1:1187003/1‑54 (MQ=255)                                                          |                                             GCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAG  >  W3110S.gb/1984300‑1984401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑