Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,985,114	A→G	G189G (GGT→GGC)	araH ←	fused L‑arabinose transporter subunits and membrane components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,985,114	0	A	G	100.0%	11.1 / NA	5	G189G (GGT→GGC)	araH	fused L‑arabinose transporter subunits and membrane components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/5);  total (0/5)

CAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG  >  W3110S.gb/1985075‑1985135                                        |                      ctgggTATTACGACAAAAGGTGGTTTTATTCAGCAGCAAGCAAAACATGATCAGACAcgcg  <  1:3053038/59‑1 (MQ=255) cAGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg  <  1:3106215/61‑1 (MQ=255) cAGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg  <  1:557257/61‑1 (MQ=255)  aGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg  <  1:295810/60‑1 (MQ=255)                  aGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg  <  1:2810685/44‑1 (MQ=38)                                        |                      CAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG  >  W3110S.gb/1985075‑1985135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑