Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,015,653	C→A	R96R (CGC→CGA)	fliF →	flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,015,653	0	C	A	100.0%	48.5 / NA	17	R96R (CGC→CGA)	fliF	flagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/2);  total (15/2)

CGGCAGATAAAGTTCACGAACTGCGTCTGCGCCTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTGC  >  W3110S.gb/2015622‑2015702                                |                                                  cGGCAGATAAAGTTCACGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAggcggc                     >  1:1937240/1‑62 (MQ=255)       aTAAAGTTCACGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGcggtc               <  1:1259056/62‑1 (MQ=255)       aTAAAGTTCACGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGcggtc               <  1:193711/62‑1 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:91519/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:742992/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:435727/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:3131642/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:2447412/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:241504/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:1785994/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:1781944/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:163810/1‑61 (MQ=255)                aCGAACTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGa       >  1:1423825/1‑61 (MQ=255)                    aCTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTgg  >  1:486324/1‑61 (MQ=255)                    aCTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTGc  >  1:2649283/1‑62 (MQ=255)                    aCTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTGc  >  1:1715553/1‑62 (MQ=255)                    aCTGCGTCTGCGACTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTGc  >  1:1507712/1‑62 (MQ=255)                                |                                                  CGGCAGATAAAGTTCACGAACTGCGTCTGCGCCTGGCACAACAAGGTTTGCCAAAAGGCGGCGCGGTCGGTTTCGAACTGC  >  W3110S.gb/2015622‑2015702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑