Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,032,239	A→T	A188A (GCA→GCT)	yedA →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,032,239	0	A	T	75.0%	15.6 / 3.5	12	A188A (GCA→GCT)	yedA	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (9/0);  total (11/1)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

TTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCG  >  W3110S.gb/2032195‑2032263                                             |                         ttacctGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGAt         >  1:1703983/1‑62 (MQ=255) ttaccgGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGAt         >  1:1799812/1‑62 (MQ=255)      tGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGt    <  1:2658639/62‑1 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGc                       >  1:1214222/1‑40 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:1609888/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:1656333/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:1682514/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:2205518/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:2466785/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:2485275/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGTCg  >  1:916546/1‑61 (MQ=255)         aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCTGGCGTGGTGTTAATGATCGCGACg  >  1:884637/1‑61 (MQ=255)                                             |                         TTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCG  >  W3110S.gb/2032195‑2032263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑