Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,039,714	A→G	G192G (GGT→GGC)	yedV ←	predicted sensory kinase in two‑component regulatory system with YedW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,039,714	0	A	G	96.5%	93.8 / ‑4.0	29	G192G (GGT→GGC)	yedV	predicted sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (0/28);  total (1/28)
Fisher's exact test for biased strand distribution p-value = 3.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTA  >  W3110S.gb/2039693‑2039754                      |                                         ataATTCAGGGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:64105/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2396021/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:979938/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:632287/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:595427/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:588344/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:567739/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:453477/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:3077332/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:3050954/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:3030203/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2768683/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2742228/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2426059/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:229581/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2172319/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2157732/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2129082/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:209369/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:2047088/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:181953/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:14598/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:1069322/62‑1 (MQ=255) ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:1064569/62‑1 (MQ=255)  taATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:1062008/61‑1 (MQ=255)  taATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:1322576/61‑1 (MQ=255)  taATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGAtt   >  1:1785702/1‑60 (MQ=255)          cgcTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:364938/53‑1 (MQ=255)             ttCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa  <  1:1576998/50‑1 (MQ=255)                      |                                         ATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTA  >  W3110S.gb/2039693‑2039754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑