Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,062,227	C→A	K275N (AAG→AAT)	cbl ←	DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,062,227	0	C	A	100.0%	101.4 / NA	32	K275N (AAG→AAT)	cbl	DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/17);  total (15/17)

CTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCG  >  W3110S.gb/2062172‑2062280                                                        |                                                      cTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGt                                                 >  1:566326/1‑62 (MQ=255) cTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGt                                                 >  1:244789/1‑62 (MQ=255) cTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGt                                                 >  1:2059774/1‑62 (MQ=255)          aaaGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCa                                         >  1:981352/1‑61 (MQ=255)          aaaGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCa                                         >  1:980267/1‑61 (MQ=255)          aaaGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCa                                         >  1:510444/1‑61 (MQ=255)          aaaGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCa                                         >  1:189065/1‑61 (MQ=255)          aaaGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCa                                         >  1:1277455/1‑61 (MQ=255)                      aGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCa                            <  1:2746497/62‑1 (MQ=255)                      aGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCa                            <  1:2558028/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:1647387/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:68713/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:347825/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:340865/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:2985159/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:172940/62‑1 (MQ=255)                               cATAGTTACGCTGAAGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGa                   <  1:1119290/62‑1 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAg     >  1:2300980/1‑62 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAg     >  1:795392/1‑62 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAg     >  1:2206855/1‑62 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAg     >  1:2202957/1‑62 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAg     >  1:1212997/1‑62 (MQ=255)                                             aGTTGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATACCGCGTATCCAg     >  1:1796881/1‑62 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:2826593/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:844946/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:68682/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:537982/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:1379492/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:2282009/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:1973144/62‑1 (MQ=255)                                                tGTCCTCGATTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCg  <  1:1731757/62‑1 (MQ=255)                                                   ccTCGATTCAGTCCCAACCAGACAGTATTAGCATCaaaaa                     >  1:2267234/1‑40 (MQ=255)                                                        |                                                      CTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGTCCCAACCAGACAGTATTAGCATCAAAAAGATGCCGCGTATCCAGGCG  >  W3110S.gb/2062172‑2062280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑