| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,065,274 | T→C | N63D (AAC→GAC) | erfK ← | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,065,274 | 0 | T | C | 100.0% | 23.4 / NA | 8 | N63D (AAC→GAC) | erfK | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/0); total (8/0) | |||||||||||
AGCATGTTACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATTGTG > W3110S.gb/2065267‑2065328 | aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:1428973/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:1498727/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:1976857/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:2526636/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:2946719/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:787945/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgtg > 1:988736/1‑62 (MQ=255)aGCATGTCACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATtgt > 1:525769/1‑61 (MQ=255) | AGCATGTTACTTAACCCTTGCCCGTATTGTGCGGCAAAAGTCTCCAGCGGCTGGGTATTGTG > W3110S.gb/2065267‑2065328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |