Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,077,046	C→T	A44V (GCC→GTC)	yeeR →	predicted membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,077,046	0	C	T	100.0%	56.6 / NA	19	A44V (GCC→GTC)	yeeR	predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/6);  total (13/6)

CACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCATAT  >  W3110S.gb/2076996‑2077094                                                   |                                                 cACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:1371055/60‑1 (MQ=255) cACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:1408258/60‑1 (MQ=255) cACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:1516047/60‑1 (MQ=255) cACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:540536/60‑1 (MQ=255) cACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:3052274/60‑1 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTActggc                                         <  1:401123/53‑1 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCtata                                 >  1:1628593/1‑61 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCtata                                 >  1:1865403/1‑61 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCtat                                  >  1:1868775/1‑60 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCTatat                                >  1:2143994/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCTatat                                >  1:814388/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCTatat                                >  1:379229/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCTatat                                >  1:2679091/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGTCTTACTGGCTGGCTatat                                >  1:1126411/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCCGTCTTACTGGCTGGCTatat                                >  1:3123574/1‑62 (MQ=255)        tCTGCGCTGGCAGGGCTAATATTGCTGTGTCTGTTCGGCCCGGTCtt                                               >  1:1335471/1‑47 (MQ=255)                                    gtCTGTTCGGCCCGGTCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCat    >  1:2735044/1‑62 (MQ=255)                                    gtCTGTTCGGCCCGGTCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCat    >  1:18381/1‑62 (MQ=255)                                      cTGTTCGGCCCGGTCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCatat  >  1:2345133/1‑62 (MQ=255)                                                   |                                                 CACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAACCCGGTTGTTCCATAT  >  W3110S.gb/2076996‑2077094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑