| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,099,935 | T→C | K168K (AAA→AAG) | cld ← | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,099,935 | 0 | T | C | 87.5% | 59.5 / 1.5 | 24 | K168K (AAA→AAG) | cld | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (0/21); total (1/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTA > W3110S.gb/2099909‑2099988 | tttttCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATc > 1:1721896/1‑62 (MQ=255) ccAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGc < 1:814421/62‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2624962/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:936538/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:896754/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:651088/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:3035644/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2997723/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2821482/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2799323/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2702709/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1060394/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2360208/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2309646/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2257330/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2154954/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2102557/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1901952/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1879787/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1877165/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1722531/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:16186/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATACTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2961433/59‑1 (MQ=255) aTGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTa < 1:2861014/62‑1 (MQ=255) | TTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTA > W3110S.gb/2099909‑2099988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |