| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,099,971 | G→A | D156D (GAC→GAT) | cld ← | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,099,971 | 0 | G | A | 77.8% | 46.6 / 10.9 | 27 | D156D (GAC→GAT) | cld | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (0/21); total (4/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
CCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTGC > W3110S.gb/2099919‑2100025 | ccAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGc < 1:814421/62‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2624962/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:936538/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:896754/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:651088/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:3035644/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2997723/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2821482/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2799323/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2702709/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1060394/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2360208/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2309646/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2257330/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2154954/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2102557/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1901952/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1879787/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1877165/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:1722531/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:16186/59‑1 (MQ=255) gaacAATGTTGTCCTTGAGATACTTTTCTAGCTCTTGATTCACTTTATCATCAACTTGCTGa < 1:2961433/59‑1 (MQ=255) aTGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTa < 1:2861014/62‑1 (MQ=255) cACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCAtt > 1:2163205/1‑43 (MQ=255) cACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCAtt > 1:2800814/1‑43 (MQ=255) cACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCAtt > 1:2852906/1‑43 (MQ=255) tttATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTGc > 1:2572205/1‑61 (MQ=255) | CCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTGC > W3110S.gb/2099919‑2100025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |