Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,208,604 T→C V225V (GTT→GTC yehL → predicted transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,208,6040TC87.0% 118.4 / 7.6 46V225V (GTT→GTCyehLpredicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base C (40/0);  total (46/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGG  >  W3110S.gb/2208544‑2208635
                                                            |                               
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt                                <  1:990157/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt                                <  1:2826358/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt                                <  1:2680262/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt                                <  1:632788/62‑1 (MQ=255)
 gATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt                                <  1:1889160/61‑1 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTa                       >  1:1339242/1‑51 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg              >  1:1017350/1‑60 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg              >  1:1993570/1‑60 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg              >  1:2396507/1‑60 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg              >  1:2251130/1‑60 (MQ=255)
                    tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg              >  1:2339313/1‑60 (MQ=255)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTc               >  1:2656996/1‑36 (MQ=255)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTc               >  1:985402/1‑36 (MQ=255)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAgct    >  1:2397641/1‑45 (MQ=255)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:395810/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2540743/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2905239/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2953371/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:3050393/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:334917/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:367589/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:957454/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:553420/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:684169/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:685455/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:699042/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:752707/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:799556/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:860991/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:924642/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2124127/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:1266094/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:1545842/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:158365/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:1600536/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:1768400/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:1796579/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:181571/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2016077/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2041489/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2793098/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2251459/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2287068/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2369525/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2531887/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2754374/1‑49 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg   >  1:2133963/1‑48 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg   >  1:461007/1‑48 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg   >  1:1379311/1‑48 (MQ=38)
                                           ttCGATTTTGAAACTGTCTTTCCGATTAAGGATTTCGCCCAGGAGCTgg  >  1:2765893/1‑49 (MQ=37)
                                           ttCGATTTTGAAACTGTCTTTCGATTATGGATTTCGCCCAGGAGCTgg  >  1:2308705/1‑48 (MQ=25)
                                                            |                               
CGATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGG  >  W3110S.gb/2208544‑2208635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: