Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,262,453	G→A	L60L (CTG→TTG)	yeiC ←	predicted kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,262,453	0	G	A	100.0%	53.8 / NA	18	L60L (CTG→TTG)	yeiC	predicted kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/0);  total (18/0)

ATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTAC  >  W3110S.gb/2262420‑2262507                                  |                                                       aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAgg                                                >  1:1308671/1‑42 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:1213925/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:932131/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:763369/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:710993/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:559776/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:474627/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:356348/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:2695058/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:2671762/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:2551378/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:2197942/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:219744/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:1728223/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:1620662/1‑43 (MQ=255) aTTGACCATAAAAATCACTGCCTACGGCGCTCAATAGCCAGGc                                               >  1:1134540/1‑43 (MQ=255)                            cgcTCAATAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTAc  >  1:2578386/1‑61 (MQ=255)                            cgcTCAATAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTAc  >  1:1749194/1‑61 (MQ=255)                                  |                                                       ATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTAC  >  W3110S.gb/2262420‑2262507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑