Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,395,203	C→T	A265T (GCG→ACG)	nuoN ←	NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,395,203	0	C	T	100.0%	81.9 / NA	28	A265T (GCG→ACG)	nuoN	NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/23);  total (5/23)

CCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACCAGCA  >  W3110S.gb/2395160‑2395250                                            |                                                ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc                               >  1:955121/1‑62 (MQ=255) ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc                               >  1:1285087/1‑62 (MQ=255) ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc                               >  1:723932/1‑62 (MQ=255)               cAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCaa                 <  1:1957020/62‑1 (MQ=255)               cAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCaa                 <  1:2389806/62‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:2436058/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:897631/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:840053/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:647468/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:581417/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:571945/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:3029574/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:290733/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:2716073/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:2701037/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:1042966/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:2316485/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:2106372/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:1812548/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:1448602/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:1197640/53‑1 (MQ=255)                                aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:104641/53‑1 (MQ=255)                                aaaCCCCTACCGTTTACATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:3041501/53‑1 (MQ=39)                                 aaCCCCTACCGTTTCCATCGACATCTCGCCGGATTGCAGCGCAATCAGCGCt         <  1:2210048/52‑1 (MQ=39)                                 aaCCCCTACCGTTTCCATCGACATCTCGCCGGATTGCAGCGCAATCAGCGCt         <  1:1980114/52‑1 (MQ=39)                                   ccccTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt         <  1:1506758/50‑1 (MQ=255)                                       tACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACcagca  >  1:2138068/1‑53 (MQ=255)                                       tACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACcagca  >  1:743191/1‑53 (MQ=255)                                            |                                                CCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACCAGCA  >  W3110S.gb/2395160‑2395250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑