Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,406,839 G→C T563T (ACC→ACG)  nuoC ← NADH:ubiquinone oxidoreductase, chain C,D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,406,8390GC76.5% 29.4 / 4.7 17T563T (ACC→ACG) nuoCNADH:ubiquinone oxidoreductase, chain C,D
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base C (0/13);  total (0/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGATCGCCGCCGGAATTTGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTGCTGCCGT  >  W3110S.gb/2406797‑2406876
                                          |                                     
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2558583/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:952301/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:592352/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:551007/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2916971/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2795816/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:1271646/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2268029/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2146324/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:2084515/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:1900474/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:1655430/61‑2 (MQ=255)
gcgGATCGCCGCCGGAATTTGCTGCAAATGCGCATAACTCGGCGTACGGATACGGGTGCga                     <  1:1624313/61‑2 (MQ=255)
                  ttGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTTCTGCCGt  <  1:1460457/62‑1 (MQ=255)
                  ttGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTGCTGCCGt  <  1:530230/62‑1 (MQ=255)
                  ttGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTGCTGCCGt  <  1:801219/62‑1 (MQ=255)
                  ttGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTGCTGCCGt  <  1:835246/62‑1 (MQ=255)
                                          |                                     
GCGGATCGCCGCCGGAATTTGCTGCAAATGCGCAAAGCTCGGGGTACGAACACGGGTGCGGTAACTCATGGTGCTGCCGT  >  W3110S.gb/2406797‑2406876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑