Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,427,821	T→C	V223A (GTT→GCT)	yfcH →	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,427,821	0	T	C	100.0%	53.3 / NA	16	V223A (GTT→GCT)	yfcH	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/9);  total (7/9)

GGTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGTTTCGCCCTACCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCAT  >  W3110S.gb/2427772‑2427870                                                  |                                                  ggTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTAccc                                       <  1:1512108/62‑1 (MQ=255) ggTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTAccc                                       <  1:2359800/62‑1 (MQ=255) ggTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTAccc                                       <  1:2824194/62‑1 (MQ=255) ggTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTAccc                                       <  1:3118116/62‑1 (MQ=255) ggTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTAccc                                       <  1:893024/62‑1 (MQ=255)              tgctgGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGa                           >  1:1749929/1‑61 (MQ=255)              tgctgGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGa                           <  1:1907651/61‑1 (MQ=255)              tgctgGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGa                           >  1:1987908/1‑61 (MQ=255)              tgctgGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGa                           >  1:3126490/1‑61 (MQ=255)              tgctgGATAACGAGCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGa                           <  1:93033/61‑1 (MQ=255)                           gCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGAAc                         >  1:1380446/1‑50 (MQ=255)                           gCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGAAc                         >  1:1806420/1‑50 (MQ=255)                           gCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGAAc                         >  1:487232/1‑50 (MQ=255)                           gCTGCGCGGGCCATTTAATATGGCTTCGCCCTACCCGGTACGCAATGAAc                         >  1:601166/1‑50 (MQ=255)                                      cATTTAATATGGCTTCGCCCTACCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCAt  <  1:3020818/62‑1 (MQ=255)                                       aTTTAATATGGCTTCGCCCTACCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCAt  <  1:1168279/61‑1 (MQ=255)                                                  |                                                  GGTATTCTCTGGCTGCTGGATAACGAGCTGCGCGGGCCATTTAATATGGTTTCGCCCTACCCGGTACGCAATGAACAATTTGCCCATGCGCTCGGTCAT  >  W3110S.gb/2427772‑2427870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑