Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,464,537	T→C	V23V (GTA→GTG)	yfcX ←	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,464,537	0	T	C	100.0%	41.7 / NA	13	V23V (GTA→GTG)	yfcX	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/12);  total (1/12)

AATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  W3110S.gb/2464480‑2464565                                                          |                             aatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACg                           >  1:1781509/1‑61 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:10661/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1073063/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1171720/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1175428/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1256032/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:2324631/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:2755977/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:362530/62‑1 (MQ=255)                         cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:745848/62‑1 (MQ=255)                          tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1968274/61‑1 (MQ=255)                          tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:431254/61‑1 (MQ=255)                          tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:676441/61‑1 (MQ=255)                                                          |                             AATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  W3110S.gb/2464480‑2464565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑