Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,515,358 G→A V95I (GTT→ATT)  yfeO → predicted ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,515,3580GA100.0% 42.7 / NA 15V95I (GTT→ATT) yfeOpredicted ion channel protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/0);  total (15/0)

CACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGC  >  W3110S.gb/2515353‑2515414
     |                                                        
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTa                          >  1:136881/1‑38 (MQ=38)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAAt                  >  1:660742/1‑46 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:1687012/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:1799461/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:2399379/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:2578931/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:2597564/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:2830495/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:2988543/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:435378/1‑62 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTgg   >  1:2703419/1‑61 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTgg   >  1:861566/1‑61 (MQ=255)
cACCGATTCCGCCCTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTgg   >  1:956685/1‑61 (MQ=255)
cACCGATTCCGCCCTCTACGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:1867373/1‑62 (MQ=255)
cACCGATTCCGCACTCTGCGCTACCAGGACTTATCGTAGCATTAATTCTCGGTCTTGCTggc  >  1:214805/1‑62 (MQ=255)
     |                                                        
CACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGC  >  W3110S.gb/2515353‑2515414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: