Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,581,387	G→A	T16M (ACG→ATG)	ypfG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,581,387	0	G	A	100.0%	61.9 / NA	20	T16M (ACG→ATG)	ypfG	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/20);  total (0/20)

AAGACGTCGGCAACAAAGCGAAAAAAAAGAGAAGGAAAATGCGATAGCGCATGGACGGTACGA  >  W3110S.gb/2581382‑2581444      |                                                          aaGACATCGGCAACAAAACG‑AAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:491684/62‑1 (MQ=37)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1226268/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1348092/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1545547/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1590977/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1092652/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1715320/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1866834/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1890780/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1909375/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:2536119/62‑1 (MQ=255)  aGACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:2896621/62‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:82105/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:759180/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:72637/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:482542/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:2521464/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:2444376/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:2016349/61‑1 (MQ=255)   gACATCGGCAACAAAACGAAAAAAAAGAGAAGGAAAATGCGATATCGCATGGACGGTACGa  <  1:1627539/61‑1 (MQ=255)      |                                                          AAGACGTCGGCAACAAAGCGAAAAAAAAGAGAAGGAAAATGCGATAGCGCATGGACGGTACGA  >  W3110S.gb/2581382‑2581444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑