| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,595,882 | C→T | M131I (ATG→ATA) | purC ← | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,595,882 | 0 | C | T | 100.0% | 124.5 / NA | 38 | M131I (ATG→ATA) | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/9); total (29/9) | |||||||||||
TACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCA > W3110S.gb/2595824‑2595937 | tACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACTAt > 1:1537955/1‑61 (MQ=255) ggTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACTATCGGATCGTGc > 1:1088759/1‑62 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAGCAGATCGaa < 1:2788859/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAGCAGATCGaa < 1:2792378/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1055141/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:539915/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:675690/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:741238/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1949564/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1415334/62‑1 (MQ=255) tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:3025584/62‑1 (MQ=255) gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTc > 1:564799/1‑61 (MQ=255) gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCa > 1:122568/1‑62 (MQ=255) gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCa > 1:568616/1‑62 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:470035/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:498192/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:478395/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2860996/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:591544/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:743628/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:75607/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:797455/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:856558/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:907127/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:464056/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2939588/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2883227/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2760998/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2739015/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2711886/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2435849/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2167179/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1747964/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1660392/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1460608/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1071571/1‑36 (MQ=255) ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAg > 1:2367371/1‑35 (MQ=255) ttGACTATCGGATCGTGCATGGCGTAGTTTTTCAgg > 1:109709/1‑36 (MQ=37) | TACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCA > W3110S.gb/2595824‑2595937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |