| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,713,103 | C→G | A292P (GCT→CCT) | yfiE ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,713,103 | 0 | C | G | 78.1% | 30.3 / 14.8 | 32 | A292P (GCT→CCT) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base G (0/25); total (3/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
AAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCC > W3110S.gb/2713044‑2713163 | aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGcc > 1:2457599/1‑61 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGcc > 1:311475/1‑61 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGcc > 1:412838/1‑61 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGcc > 1:1551501/1‑61 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGcc > 1:167142/1‑61 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGc > 1:1320004/1‑60 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCca < 1:2897559/62‑1 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCca < 1:2159996/62‑1 (MQ=255)aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCca < 1:1330319/62‑1 (MQ=255) aTGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGa < 1:1936356/61‑1 (MQ=255) gACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGcc > 1:1682333/1‑62 (MQ=255) gACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGcc > 1:1771452/1‑62 (MQ=255) gACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGcc > 1:2037340/1‑62 (MQ=255) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:919816/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:2969770/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:3018675/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:3089010/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:902450/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:366893/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:862172/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:769811/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:43854/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:531160/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:573879/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:710595/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:743118/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:2838139/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:2772210/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:2383550/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:2197018/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1986063/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1939946/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1938081/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1868392/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1686211/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:138302/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1355823/39‑1 (MQ=19) tgtttttATCCCGGCAAAAAACACTCTTCAACACACTGAAt < 1:1339025/39‑1 (MQ=19) ccACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTcc > 1:2671623/1‑61 (MQ=255) ccACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTcc > 1:1727518/1‑61 (MQ=255) ccACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTcc > 1:1115923/1‑61 (MQ=255) | AAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCC > W3110S.gb/2713044‑2713163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |