Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	549,463	C→A	T205N (ACC→AAC)	ylbF →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	549,463	0	C	A	78.6%	19.1 / 2.8	14	T205N (ACC→AAC)	ylbF	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (0/11);  total (0/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGACAATCTGCAACTCGTTACCACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGAT  >  W3110S.gb/549442‑549507                      |                                             ccGACAATCTGCAACTCGTTACCACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCa       <  1:3030984/61‑1 (MQ=255) ccGACAATCTGCAACTCGTTACCACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCa       <  1:594778/61‑1 (MQ=255) ccGACAATCTGCAACTCGTTACCACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCa       <  1:989285/61‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:118059/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:1264984/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:1520961/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:154747/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:1710670/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:1737110/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:2018093/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:2092528/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:2551792/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:2643176/62‑1 (MQ=255)     cAATCTGCAACTCGTTAACACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGat  <  1:2654193/62‑1 (MQ=255)                      |                                             CCGACAATCTGCAACTCGTTACCACAGCGGTGAGCGTCAGTTATTTACGTTATGCCGCGCAAGGAT  >  W3110S.gb/549442‑549507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑