Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,815,156	G→A	intergenic (‑61/+12)	gshA ← / ← yqaA	gamma‑glutamate‑cysteine ligase/conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,815,156	0	G	A	100.0%	50.5 / NA	17	intergenic (‑61/+12)	gshA/yqaA	gamma‑glutamate‑cysteine ligase/conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/9);  total (8/9)

GCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAAC  >  W3110S.gb/2815107‑2815209                                                  |                                                      gCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTACAGGCCTACAAt                                           <  1:965/62‑1 (MQ=255)               ttttAATTAGCATAACTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCCCCAcat                             >  1:349538/1‑62 (MQ=255)               ttttAATTAGCATAACTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCAcat                             >  1:1552328/1‑62 (MQ=255)               ttttAATTAGCATAACTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCAcat                             >  1:2748159/1‑62 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:871687/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:1466613/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:510637/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:453777/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:2024561/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:2462286/62‑1 (MQ=255)                             aCTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:2983866/62‑1 (MQ=255)                              cTGTAATGGTGACCATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACgg               <  1:113213/61‑1 (MQ=255)                                          ccATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGctgcaac  >  1:2420148/1‑62 (MQ=255)                                          ccATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGctgcaac  >  1:1988222/1‑62 (MQ=255)                                          ccATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGctgcaac  >  1:1665663/1‑62 (MQ=255)                                          ccATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGctgcaac  >  1:1388708/1‑62 (MQ=255)                                          ccATATGTACAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGctgcaac  >  1:1326660/1‑62 (MQ=255)                                                  |                                                      GCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAAC  >  W3110S.gb/2815107‑2815209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑