Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,845,313	T→C	G252G (GGA→GGG)	hycD ←	hydrogenase 3, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,845,313	0	T	C	100.0%	11.9 / NA	5	G252G (GGA→GGG)	hycD	hydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/4);  total (1/4)

AGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCC  >  W3110S.gb/2845278‑2845344                                    |                                aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa                   <  1:1338117/50‑1 (MQ=255) aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa                   <  1:2945369/50‑1 (MQ=255) aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa                   <  1:3096580/50‑1 (MQ=255)       aCGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGAAGGTTTCCATTTGTcccc  <  1:542095/61‑1 (MQ=255)        cGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGAAGGTTTcc            >  1:706500/1‑50 (MQ=255)                                    |                                AGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCC  >  W3110S.gb/2845278‑2845344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑