Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,866,110	T→A	intergenic (‑20/+160)	rpoS ← / ← nlpD	RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,866,110	0	T	A	100.0%	70.7 / NA	23	intergenic (‑20/+160)	rpoS/nlpD	RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/0);  total (23/0)

CTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACT  >  W3110S.gb/2866079‑2866145                                |                                    ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2458280/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:991726/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:681829/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:659682/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:652217/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:635725/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:607272/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2892445/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2777689/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2776898/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2586767/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1072384/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2336610/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2114954/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:2112595/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1898079/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1589860/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1586632/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1527362/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1302469/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTc                                 >  1:1213222/1‑36 (MQ=255)      cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACt  >  1:386853/1‑62 (MQ=255)      cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACt  >  1:1736088/1‑62 (MQ=255)                                |                                    CTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACT  >  W3110S.gb/2866079‑2866145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑