Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,871,070	G→A	P26L (CCT→CTT)	ispD ←	4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,871,070	0	G	A	100.0%	37.0 / NA	13	P26L (CCT→CTT)	ispD	4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

CGCATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAAC  >  W3110S.gb/2871015‑2871113                                                        |                                            cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:1097302/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:16740/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:1811099/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:1965177/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:2146211/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:689645/62‑1 (MQ=255) cgcATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAAGACAtt                                       <  1:906702/62‑1 (MQ=255)                            ttGATTACCGATTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCCAAATCCgg            <  1:830787/62‑1 (MQ=255)                                ttACCGATTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCCAAATCCGgccg         <  1:1457856/61‑1 (MQ=255)                                      aTTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCGAAATCCGGCCGCCGGAAc  >  1:846501/1‑62 (MQ=255)                                      aTTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAAc  >  1:1878826/1‑62 (MQ=255)                                      aTTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAAc  >  1:1966217/1‑62 (MQ=255)                                      aTTGAGAGATATTGCTTAAGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAAc  >  1:248149/1‑62 (MQ=255)                                                        |                                            CGCATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAAC  >  W3110S.gb/2871015‑2871113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑