Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,910,291	C→T	A673T (GCT→ACT)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,910,291	0	C	T	100.0%	123.0 / NA	38	A673T (GCT→ACT)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/14);  total (24/14)

ACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCG  >  W3110S.gb/2910270‑2910344                      |                                                      acaacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGct                >  1:2480284/1‑61 (MQ=255) acaacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGct                >  1:2417518/1‑61 (MQ=255) acaacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGc                 >  1:726049/1‑60 (MQ=255)   aacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCtctc             <  1:520803/62‑1 (MQ=255)   aacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCtctc             >  1:3070619/1‑62 (MQ=255)   aacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCtctc             <  1:2778869/62‑1 (MQ=255)   aacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCtctc             <  1:2713815/62‑1 (MQ=255)   aacCCACTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCtctc             >  1:1886948/1‑62 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1111747/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:623188/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:382821/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:345605/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:3086122/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:257411/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:2237857/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:2169207/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:2097077/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1108160/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1239738/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1537061/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:2111245/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1721741/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAccc         >  1:1713054/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCAcc          >  1:1836191/1‑60 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCGAATATCCGCCGGAGTAGCTCTCAccc         >  1:170370/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCAAATATCCGGCGGAGTAGCTCTCAccc         >  1:786787/1‑61 (MQ=255)        aCTACGATCATTAGTTACCACGCGGACCACCAGCAAATATCCGGCGGAGTAGCTCTCAccc         >  1:790377/1‑61 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:3054116/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:2959240/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:1768104/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:316140/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:177162/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:363676/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:146763/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:1837111/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:1217701/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:2053777/62‑1 (MQ=255)              aTCATTAGTTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACcg  <  1:980778/62‑1 (MQ=255)                      |                                                      ACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCG  >  W3110S.gb/2910270‑2910344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑